Bio-Rad Bio-Plex Software® Upgrades and Conversions User Manual

Page 130

Advertising
background image

Bio-Plex Manager Software 6.1 User Guide | Running Protocols

118

D

ENSITY

F

ILTERING

Each colored dot in the bead map represents a certain number of events
measured by the reader at that data point. Different colors are used to
distinguish among different numbers of events. You can adjust the display so
that data points with low numbers of events are not shown, or to set the
number of events at before which the data point color changes.

Figure 106. Bead map display options

NOTE: These settings only change the bead map display, and do not affect
the underlying data in any way.

First, enter a number in the Level Multiplier field. This number is used
exponentially to determine the number of events required before a data point
changes color. For the default value of 2, the color level changes at 2, 4, 8, 16,
etc. events.

If you want to filter lower numbers of events from the display, select the Filter
Levels checkbox and use the arrow keys next to the Display Above Level field
to select the exponent level below which no data points will be displayed. For
example, if the Level Multiplier is set to 2, and the Display Above Level field is
set to 3, data points are not displayed until 8 or more events are registered;
the first color level occurs at 8 events, the second color level occurs at 16
events, the third color level occurs at 32 events, etc.

The color levels in the display are, in ascending order, dark blue, purple, dark
green, light blue, blue, light green, orange, black, and brown.

L

OGARITHMIC

/L

INEAR

D

ISPLAY

Select whether you want to display the bead map data on a logarithmic or
linear scale using these option buttons.

BioPlex_6.book Page 118 Friday, September 23, 2011 11:55 AM

Advertising