Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 226)

Bio-Plex Manager Software 6.1 User Guide | Data Normalization
214
Normalization Factor Calculations
Normalization factors are determined in the following ways.
Table 1.
Case Factor Definition
A Control sample
present for
normalization Average of (FI-Bkgd) for the external control
for all analytes.
Factor = Average [
(FIcontrol-Bkgdcontrol) analyte1,
(FIcontrol-Bkgdcontrol) analyte2,
(FIcontrol-Bkgdcontrol) analyte3, etc.]
B No control sample
and single
housekeeping gene Average of (FIhk-Bkgdhk) for all wells with this
housekeeping gene. Data are taken from raw
data table (average replicate values for each
sample type from report table are not
included).
Factor = Average [
(FIhk-Bkgdhk) well1,
(FIhk-Bkgdhk) well2,
(FIhk-Bkgdhk) well3, etc.]
C No control sample
and multiple
housekeeping
genes Factor = Geometric Mean (Factorhk1,
Factorhk2, Factorhk3, etc.)
BioPlex_6.book Page 214 Friday, September 23, 2011 11:55 AM