Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 108)

Bio-Plex Manager Software 6.1 User Guide | Preparing Protocols
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Step 5. Enter Controls Info (Optional)
Controls are samples of known concentration whose values are measured
during a reading in the same manner as the unknown samples. You can then
compare the calculated concentrations of the controls after a reading with the
expected concentrations to determine the overall accuracy of the assay. (This
is sometimes called the "spiked" recovery method, because you are "spiking"
your assay with known quantities of analytes.)
In this step, you enter the concentration values of your controls. First, you
must select the analytes you will be analyzing in the reading as described on
page 59, and identify the plate well(s) containing your controls as described
on page 73.
NOTE: Only formatted wells are read by the array reader. Be sure to define all
your control wells before running a protocol.
Click the Enter Controls Info button in the Protocol window to enter
information about your controls.
Figure 88. Enter Controls Info dialog
The analytes you selected in the Select Analytes window are available under
the Analyte pulldown menu. Select the first analyte for which you want to
enter controls.
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