Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 44)

Bio-Plex Manager Software 6.1 User Guide | Controlling the System
32
Next, enter the DD, CL1, and CL2 target values for the control number, as
printed on the bottle, in the appropriate fields.
NOTE: Use only the DD target value on the Bio-Plex CAL1 bottle. Using
Bio-Rad’s calibrators is highly recommended, because other manufacturers’
DD target values have not been validated.
Click Add to close the dialog box and save your changes.
To add a new CAL2 control number, click the Add button under CAL2 Control
Number in the Calibrate dialog box. The Add New CAL2 Control Number
dialog box opens.
Figure 18. Adding a new CAL2 control number
In the dialog, enter the control number from the CAL2 bottle in the field. Select
the expiration date, if printed on the bottle, as described above. Next, enter
the Low RP1 target value for the control number, as printed on the bottle, in
the RP1 field.
NOTE: The CAL2 calibration bottle label lists two RP1 (also known as PMT, for
photomultiplier tube) target values: Low RP1 and High RP1. The High RP1
target value cannot be used in Bio-Plex Manager 5.0 or higher. All calibration
is done at the Low RP1 target value, which must fall between 3000 and 4000.
The High RP1 target value remains on the bottle label because it can be used
with earlier versions of Bio-Plex Manager. Assays that require calibration using
the High RP1 target value can be run by selecting the checkbox labeled “Run
at High RP1 Target” in the Run Protocol window.
When you are done, click Add to close the dialog box and save your changes.
Your new control numbers are added to the selection lists in the main
Calibrate dialog.
To delete a particular control number, first select it in the Calibrate dialog box,
and then click the Delete button.
BioPlex_6.book Page 32 Friday, September 23, 2011 11:55 AM