Method development using sitechart – INFICON Explorer Portable Gas Chromatograph User Manual

Page 162

Advertising
background image

Method Development

160

Method Development Using SiteChart

Assay development can be approached in two ways: first, you can develop a custom
assay based on the compounds that are included as part of the assay that has been
shipped with your instrument. Second, you can develop your own library of
compounds.

If you use the first method, all the required chromatographic settings for each
compound are stored and the only steps required are selecting the compounds of
interest and calibrating the instrument. Each library listing has been setup for a
specific application. The compound groups and the associated chromatographic
settings have been selected to obtain the best results. Refer to the assay notes for
details.

If you use the second method, you have complete control of the GC. You can adjust
any of the chromatographic settings, such as temperature and flow rate and optimize
the analysis for your compounds of interest. However, you must enter all your
compound information, as the information in the reference library becomes invalid.
Once your method has been entered you can save it and update the information as
necessary.

Library

The library is specific to an assay. If you have obtained Assay 1, for example, then
you will have a library of approximately 40 compounds from which you can develop
your custom assay. The library is loaded as part of the assay.

A library is supplied for each column/detector pair required for the assay. The
number of libraries is determined by the assay. The compound information in the
libraries has been determined by the standard method. The libraries are always
available on the assay disk so you can use the original method and the information
in the reference library.

Advertising