6 ethanol rotation, Operation – Leica Biosystems ASP6025 User Manual

Page 106

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Instructions for Use V 2.2 RevI – 08/2013

5.2.6 Ethanol rotation

Functional description

Ethanol has to be replaced while a program is running.
For this to be carried out, CONCENTRATION mode must be turned on the PROGRAM OPTIONS field in the SYSTEM SETUP
window, see

Chapter 5.1.2

.

In this case, the system bottles for ethanol (S1-S6) must be filled according to the pattern shown in

Fig. 141

. In addition, all

six ethanol stations must be defined in the program. The program checks this when it is started. If there is a discrepancy, a
warning message appears and the program cannot be started.

In CONCENTRATION mode, the system
bottles that are filled with ethanol in row
S1-S6 are used.

Important!
Filling of all bottles (system bottles and
RTU-bottles) with reagents must be car-
ried out exactly as shown in

Fig. 143

.

Fig. 143

RTU-bottle D1: Formalin
RTU-bottle D2: Process water
RTU-bottle D3: Xylene
RTU-bottle D4: 100 % ethanol
RTU-bottle D5: Cleaning xylene
RTU-bottle D6: Cleaning alcohol

System bottle S1: 70 % ethanol
System bottle S2: 80 % ethanol
System bottle S 3: 95 % ethanol
System bottle S4-6: 100 % ethanol
System bottle S7-9: Xylene

5. Operation

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