Leica Biosystems ASP6025 - Manual (Page 172)

170 Instructions for Use V 2.2 RevI – 08/2013
7. Troubleshooting
7.3 Typical fill or drain problems
Fill and drain problems may be due to several causes:
1st There is insufficient reagent
• Verify that the fill level in the system bottles, RTU-bottles and paraffin containers is
sufficient.
2. Insufficient pressure or vacuum
The instrument is unable to create sufficient vacuum (for a fill) or pressure (for a drain) in the
retort.
• Check whether the system bottles and RTU-bottles concerned have been inserted
correctly.
• Verify that all system bottles are fully pushed home into their sockets.
• Check whether dirt deposits have collected under the retort seal.
3. There is a blockage in the air or reagent lines
Blockages in the reagent lines are typically caused by paraffin or tissue debris. If it is not
possible to drain the reagent in the retort, try to remove the blockage as follows:
• Heat the retort to the maximum possible temperature (consistent with the reagent cur-
rently in the retort, if any). Leave the retort at this temperature for at least 15 minutes.
• When the retort reaches maximum temperature, attempt to carry out a filling and drain-
ing cycle.
• If there is no reagent in the retort:
Clean the retort in a cleaning program (
see Chapter 6.1 ).
If you cannot determine or eliminate the problem by carrying out the above
listed steps, call Leica Technical Service before attempting to run any further
programs.