Leica Biosystems ASP6025 - Manual (Page 145)

143 Leica ASP6025
5. Operation
5.6.3 Xylene-free infiltration programs
Reagent positioning/Replacement cycle
for time-optimized xylene-free programs:
System bottle S1 contains 70 % ethanol.
System bottle S2 contains 85 % ethanol.
Each program also requires an additional step with ParaLast to reduce reagent carry-over.
The pressure/vacuum cycle requires a minimum step duration of 6 minutes!
Bottle Reagent
S1 70 % ethanol
S2 85 % ethanol
S3 -empty-
S4 80/20 ethanol/isopropanol
S5 80/20 ethanol/isopropanol
S6 100 % isopropanol
S7 100 % isopropanol
S8 100 % isopropanol
S9 - empty -
D1 Formalin
D2 -empty-
D3 ParaLast
D4 -empty-
D5 Cleaning xylene
D6 Cleaning alcohol
Xylene-free infiltration programs cannot be renamed, edited or deleted by the user.
To start a xylene-free program, it must have been saved in the list of "FAVORITES". See also Chapter 5.5.3 .
The specified isopropanol concentrations are the starting concentrations that apply for the installation of the
ASP6025 modular tissue processor.