Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 104)

Section 6 Data Analysis Module
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arrow buttons to move the member to the desired position within the list of names. More
than one Gene or Condition Name can be selected (highlighted) by clicking on the
desired names. Note that when the Manual order radio button is active, a total of four
arrow buttons are displayed. The outermost arrow boxes move the selected items to the
top or bottom of the list, while the innermost arrow buttons allow for stepwise movement
of the Gene or Condition Name
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When finished sorting, click OK to view the changes to the chart
Fig. 6.56. The Sort Options Dialog Box.
Corrected Std Devs
The Corrected Std Devs selection enables the display of error correction propagated from the
standard curve data. This option should only be used if a standard curve was created as part of
the gene expression analysis. The resulting error correction is displayed as a property of the
individual error bars on the chart. The exact error value can be displayed by mousing over the
error bars to reveal the tool tip, or by displaying the Data Table and then selecting Show Details.
When Corrected Std Devs is active, a check mark is displayed next to its name in the gene
expression chart context menu.
Gene Expression Chart Tool Tips
Tool Tips are visible on the gene expression chart when the mouse cursor is positioned on one of
the chart data bars or error bars.
When the mouse cursor is positioned on the chart data bars, the resulting Tool Tip will display
the data label (that is, Gene or Condition Name, depending on which x-axis mode is selected)
along with the calculated Expression value in parenthesis.
When the mouse cursor is positioned on the chart error bars, the resulting Tool Tip will display
the Condition Error or Gene Error, depending on which x-axis mode is selected. The error value
displayed is the Unscaled Expression standard deviation. However, the Tool Tip will display the
Corrected Unscaled Expression standard deviation if Corrected Std Devs is selected from the
Gene Expression context menu.
Copy Graph/Export a Graph
To export a graph:
1. Place the mouse pointer over the graph.
2. Right-click on the graph. A shortcut menu appears.