Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 94)

Section 6 Data Analysis Module
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1. Activate the copy command by first selecting the wells to be copied from the plate
interface.
2. Right-click on the plate interface to reveal the Copy Gene or Copy Condition options,
and select the desired option.
3. Once the Copy Gene or Copy Condition option has been selected, the Paste
Gene/Condition Name text from the menu becomes active (no longer grayed out).
4. Return to the plate interface to select the destination wells for the copied
identification information.
5. Right-click on the plate interface to reveal and select the Paste Gene/Condition Name
option.
• Show Sample Type: The Show Sample Type option is selected by default. This
selection is illustrated by a check mark shown before the text in the menu. Clicking on
Show Sample Type toggles the display of the Sample Type in the plate interface.
Choosing to remove the sample type information from the plate interface simply allows
visibility of a greater number of wells in the plate interface grid.
•
Print: The Print command, on the plate interface menu, will print the displayed plate
interface.
•
Export to Excel: The Export to Excel command is useful for exporting exact values from
the plate interface. When the Export to Excel command is selected from the menu, the
iQ5 software exports the selected data into a protected workbook that is automatically
open by Microsoft Excel. The protected workbook contains all of the formatting and
fluorophore information separated into worksheets.
NOTE: The numeric values contained in the protected workbook are exact values from
the software application that include several non-significant figures beyond the decimal
point. This is important to note when considering whether to transfer spreadsheets by a
copy and paste command or the Export to Excel command. With the copy and paste
command, only the significant digits displayed in the iQ5 software interface are
transferred to Excel.
6.9.4 Setting Analysis Parameters for Gene Expression Analysis Using
the Settings Tab
The Settings tab is used to assign gene and conditions parameters and efficiency values to be
used in the calculations for gene expression analysis. There are three sections of the Settings
options window:
•
Gene List
• Condition List
• Data Set List
6.9.4.1 Gene List: Assigning Reference Genes and Target-Specific Reaction
Efficiency
When the Settings button is active and Gene List is selected, the genes being analyzed in the
experiment are listed along with their full name. This Full Name column is editable so that longer,
more descriptive names may be entered if your Gene Name entry was abbreviated.
NOTE: Gene Name entries have a character entry limit of 15 characters.