Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 82)

Section 6 Data Analysis Module
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more wells on the plot or in the spreadsheet and then return to Automatic Call and the
software will position the vertical and horizontal bars based on the new definitions, and
then make genotype assignments.
6.7.5 Manual Calls
To make manual calls on the plot:
1. Click Manual Call. A new box appears in the window (Figure 6.32).
Fig. 6.32. The Manual Call Specification Options Box.
2. Select one of the allelic call types by clicking its radio button.
3. On the plot, drag the cursor around the well or wells to be assigned the call. When the
mouse button is released, the new assignment will be made on both the plot and the
data spreadsheet.
To make manual calls in the data spreadsheet:
1. Click Manual Call.
2. Click the cell for the desired well in the Call column of the data spreadsheet. Select the
new call from the pull-down menu.
3. Note that “unknown” may only be selected in Manual Call mode.
6.7.6 Display Mode
Choose the aspect of the data that will be analyzed by clicking either Threshold Cycle or RFU
(Figure 6.33).
Fig. 6.33. The Display Mode Option Buttons.
When you click RFU, the Select Cycle option (Figure 6.34) appears so you can analyze RFU data
from any cycle. Change the cycle by entering a new value in the Select Cycle drop-down list.