Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 95)

Section 6 Data Analysis Module
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To access the gene list:
1. Make sure the Settings button is activated. If not, click Settings. See the example in
Figure 6.42.
2. Click Gene List.
The list of genes appears in the spreadsheet directly below the Gene List option button as shown
in Figure 6.42.
Fig. 6.42. Selecting the Gene List Option.
Assigning a Reference Gene
To assign a reference gene:
1. With the Settings button active, select Gene List.
2. Select the checkbox in the Ref column within the control condition(s) to which you want
to assign a reference gene. You can assign the role of reference gene to as many genes
as you wish. In Figure 6.43, Actin and Gapdh have been selected as reference genes.
Fig. 6.43. Assigning a Reference Gene.