Bio-Rad iQ™5 Optical System Software, Version 2.1 User Manual

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Section 4 Workshop Module

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4.1.1 File Browser Section

Located in the top left of the Setup window, the file browser area contains a folder tree on the
left side and a file list on the right side.

The files displayed in the list depend upon the selected Setup window tab. With the Setup tab
active, the displayed Setup window contains four additional tabs:

Protocol: Used to select, edit, or create a protocol for running a real-time experiment.
If the Protocol tab is selected, the iQ5 software displays protocol files, file extension
.tmo. The selected protocol appears in the Selected Protocol window

Plate: Used to select, edit, or create a plate setup for running a real-time experiment. If
the Plate tab is selected, the iQ5 software displays plate setup files, file extension .pts.
The selected plate appears in the Selected Plate Setup window

Run Set: Consists of a linked protocol and plate setup, useful when you repeat the same
experiment on a regular basis. If the Run Set tab is selected, the iQ5 software displays
run set files with the extension .run. The selected run set, which is a linked protocol and
plate setup, is displayed in the Selected Protocol and Selected Plate Setup windows

Data File: Primarily used to select and open a data file. You can also use this tab to run
a real-time experiment using the same protocol and plate setup that were used to create
the data file. The selected plate setup can be either the Original or Current (last saved)
plate setup. If the Data File tab is selected, the iQ5 software displays data files created
from previous experimental runs, which have the file extension .opd. The iQ5 software
displays the selected data file name and any associated notes in the Selected Data File
window. The Notes box is editable only after you open the data file. The iQ5 software
displays the protocol and plate setup used in creating this data file in the Selected
Protocol and Selected Plate Setup windows, respectively

4.1.2 Selected Protocol Area

Located in the bottom left of the Setup window, the Selected Protocol area displays the details of
the protocol selected in the file browser area. The selected protocol file name appears at the top
of this window. If a data file is selected, the iQ5 software displays the protocol used to create the
data file and the data file name. The selected protocol is displayed both graphically and in
spreadsheet format.

4.1.3 Selected Plate Setup Area

Located in the bottom right of the Setup window, the Selected Plate Setup window (Figure 4.2)
displays the details of the plate setup selected in the file browser area. The selected plate setup
name appears at the top of this window. If you have selected a data file, then the plate setup
within the data file and the data file name are both selected in the iQ5 software.

When you select the Data File tab and a data file, the iQ5 software displays the Original and
Current plate setup. The Original plate setup is the plate setup used to create the data file. The
Current plate setup is the plate setup last saved with the data file. If you are looking at a data file
that contains a plate setup that was edited and saved after the experimental data were collected,
you can revert to the original plate setup by clicking Original in the Plate box. To go back to the
present definition, click Current.

None of the information displayed on the plate in the Workshop home window can be edited
from this screen.

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