Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 36)

Section 4 Workshop Module
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The Horizontal and Vertical Replication buttons, together with the number in the
Size box, specify the direction and number of replicates to be automatically
defined.
With the horizontal direction (row) button active and the Size set at 5, if you:
•
Click a well, the sample type is incremented by one for the next five wells
in the horizontal direction, and each of the five wells will contain an
identical replicate number. If there are not five wells left in the row, the
software will wrap around to the next row
•
Click a row letter, the sample number of the first five wells will be
incremented by one, the next five wells incremented by two, and the last
two wells will be incremented by three. The software does not
automatically wrap around so that the last set of replicates has the same
size as the others
•
Drag across a selection, the first five members of the selection are
incremented by one, the second five members are incremented by two,
etc. Numbering goes in a horizontal direction and wraps around to the
next row within the selection. The last set of replicates may have a
smaller size than the others, depending on the number of wells within
the selected area
With the Vertical direction (column) button active and the Size set at 3, if you:
•
Click a well, the sample type is incremented by one for the next three
wells in the vertical direction. If there are not three wells left in the
column, it will wrap around to the next column
•
Click a column number, the sample number of the first three wells will be
incremented by one, the next three wells incremented by two, and the
last two wells will be incremented by three. The software does not
automatically wrap around so that the last set of replicates has the same
size as the others
•
Drag across a selection, the first three members of the selection are
incremented by one, the second three members are incremented by two,
etc. Numbering goes in a vertical direction and wraps around to the next
column within the selection. The last set of replicates may have a smaller
size than the others, depending on the number of wells within the
selected area
The Next # box allows you to overwrite the default replicate number for the
next sample to be defined. This is an easy way to correct a mistakenly assigned
replicate number. Use the up and down arrows to select the next desired
replicate number, or highlight the field and type the desired number.
4.2.7 Sample Volume, Seal Type, and Vessel Type
In Plate Setup you must specify the sample volume, the type of sealing, and the type of vessel to
be used in the experiment. This is to properly associate the appropriate calibration files unique
for each vessel and sealing combination with the data file. Use the up and down arrow keys to
specify the sample volume, or highlight the field and type in the volume. Select the seal type and
vessel type from the pull-down menus.