Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 55)

Section 5 Run-Time Central Module
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Add 10 Repeats: Click Add 10 Repeats to add additional repeats to the current cycle.
You can click this button multiple times, however, the total number of repeats is limited
to 600. For example, it may be necessary to add repeats to a run in an experiment
amplifying low copies of DNA to allow all samples to cross threshold. Click Add 10
Repeats to an amplification cycle of 30 repeats to make it 40 repeats. Modifications to
the protocol are updated on the protocol displayed on the iCycler base module
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Pause/Stop/Resume Run: The Pause/Stop Run allows you to pause a thermal
cycling protocol. If you click Pause/Stop Run when the iCycler thermal cycler is at a
setpoint temperature, the instrument will hold at the setpoint temperature and stop
counting down the dwell time. If you click Pause/Stop Run when the iCycler cycler is
ramping to the temperature, the instrument will continue ramping until it reaches the
next setpoint temperature, then pause at that step. Clicking Pause/Stop Run activates
two new buttons. Click Resume Run to resume the thermal cycling protocol. Click End
Run to terminate the experiment