Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 57)

Section 6 Data Analysis Module
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Gene Expr: The Gene Expression screen has flexible tools for the determination of the
fold induction of one gene relative to another gene or relative to itself under different
circumstances, for example, temporally, geographically, or developmentally different
points
• Edit Plate: In the Edit Plate screen, you may make changes to the sample type
assignment or to the quantities of the Standards in the plate setup used to run the
experiment. This is a simple way to salvage your experiment should you make mistakes
in the sample type assignment. While you may make changes to the plate setup, the
original plate setup is never discarded and always remains with the data file so that you
may revert to it at any time
6.1 PCR Quant Tab
The PCR Quant tab is the tab that is first displayed after opening a data file that contains
amplification data (Figure 6.1). For experiments that lack amplification data, such as Melt Curve
only or End Point Only experiments, the PCR Quant tab is grayed out and unavailable for
analysis.
Use the PCR Quant tab to set the analysis conditions for the data file. The analysis conditions
include setting the PCR baseline, setting the threshold, and determining which wells to exclude or
include in the experiment. The analysis conditions should be set before using the Gene
Expression, End Point, or Allelic Discrimination tabs.
For experiments with standards of known quantities, the PCR Quant tab is also where you can
determine absolute quantities for unknown samples. You can also determine the efficiency of the
PCR reaction using standard curves with either known quantities used to produce the standard
curve, or by using a serial dilution of the template under investigation.
Fig. 6.1. The PCR Quant Tab.