Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 105)

Section 6 Data Analysis Module
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3. Click Copy Graph.
4. Switch to or open the document into which you will paste the graph.
5. Paste the graph image by choosing Edit, then Paste, or by pressing CTRL+V.
6.9.8 Normalized Expression Calculations
The normalized expression calculation is stated below. To see how the iQ5 software calculates
relative quantities, go to relative quantity calculations.
Normalized Expression when a Control is Chosen
When a control is chosen, the iQ5 software uses the equation listed above to calculate
normalized expression. If a control is assigned, relative quantities for all genes within the control
sample are equal to 1. This results in “normalized” expression of 1 for the control, and relative
quantities for all other conditions will be presented relative to this normalized control sample.
According to the calculations performed by the iQ5 software, normalized expression is equivalent
to unscaled normalized expression analysis when a control is chosen.
Normalization Factor
The denominator of the normalized expression equation is referred to as the normalization factor.
The normalization factor is the geometric mean of the relative quantities of all the reference
genes for a given sample.
Scaled Normalized Expression
Scaling to highest or lowest is achieved simply by dividing all unscaled normalized expression
values for a given gene by the highest or lowest expresser respectively.
Scaled to Highest
Scaled to Lowest