Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 77)

Section 6 Data Analysis Module
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Fig. 6.25. A Melt Peak Chart.
6.6.4 Melt Curve/Peak Control Area
The control area appears between the spreadsheet and the plots. This area contains buttons to
access the Display Wells and Analyze Wells dialog boxes and the Restore Defaults function
(Figure 6.26).
Fig. 6.26. The Control Area.
The position of the temperature bar, threshold bar, and the height and well of the currently
selected peak are also displayed here. You cannot edit any of these fields. When the Edit melt
peak begin/end temp option is active, the most-recently modified temperature (begin or end) is
displayed in the Temperature Bar field.
6.6.5 Melt Curve/Peak Spreadsheet
The spreadsheet below the plots displays information about the melt peaks, the RFU data, or the
–d(RFU)/dT data for each well (Figure 6.27). None of the fields may be edited, though you may
eliminate peaks from the spreadsheet by either highlighting the peak in the spreadsheet and
clicking Delete Selected Peak, or by dragging the threshold bar up to a position above the peak.
Fig. 6.27. The Melt Peaks Spreadsheet.