Bio-Rad iQ™5 Optical System Software, Version 2.1 User Manual

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Section 1 Getting Started

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1.2.3 Installing the Support Bracket

A support bracket with roller is provided for the MyiQ2, MyiQ, or iQ5 optics module. It is mounted
to the rear of the iCycler thermal cycler.

Align the optics module support bracket with the two holes on the rear of the iCycler thermal
cycler.

Using a #2 Phillips screwdriver, adjust the height of the bracket with two of the appropriate
screws provided with the system accessories. Both of the screws should be approximately in the
center of the slots on the bracket.

1.2.4 Installing the Optics Module

1. Remove the plastic sheath and protective label from the optics module and place the

optical module on a flat surface, taking care not to touch the inside of the nose portion of
the module.

2. Remove the protective label from the optical lid.

3. Slide the optics module onto the U-bracket.

4. Secure the optics module to the U-bracket using the two long, thin hex screws and the

hex driver provided.

5. After the optics module has been installed, confirm that the optics module and lid

assembly can be opened and closed readily.

If the lid is difficult to open, lower the support bracket slightly before tightening the bracket
mounting screws. If the lid is difficult to close, try raising the support bracket slightly before
tightening the rear screws.

1.2.5 Connecting Power and Communication Cables to the System

Before connecting any communication or power cables to the system, confirm that the power
switch for the iCycler thermal cycler and the optics module are in the OFF position.

1. Close the optical reaction block by sliding the lid forward and pressing down on the lid

handle. On the right side of the optics module are three connectors.

2. Using the cables provided, establish power and communication with the computer as

follows:

Recessed 3-pin power connector — Connect the supplied power cord between the
optics module and a grounded power outlet. This connection provides power only to
the optical module; a separate power cord must be connected to the iCycler thermal
cycler

Serial connector — A serial connector is located at the rear of the iCycler thermal
cycler. Connect the serial cable to the rear of the iCycler chassis and to the serial port
on the side of the optics module. This connection enables communication between the
iQ5 software and the iCycler thermal cycler

USB port connector — Connect the supplied USB cable between the optics module and
a USB 2.0 high-speed enabled port on the computer. Data are transferred to the
computer via this cable. This single connection directs the operation of both the optics
module and the iCycler thermal cycler by the iQ5 Optical System software

At the right rear corner of the optical reaction block is a single connector the Positive
docking connector — This self-aligning connector is secured into place when the optics

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