Bio-Rad iQ™5 Optical System Software, Version 2.1 User Manual
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Section 7 Calibrating the Instrument
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To perform a pure dye calibration run:
1. Select from the Pure Dye Plate Setup file selector window or click Create New. Ensure
appropriate dyes are selected that are compatible with the filter setup of your connected
instrument. If incompatible dyes are selected the following error will appear.
2. Click Run Pure Dye Calibration.
3. When the iQ5 software completes the pure dye calibration run, a dialog box appears with
the message, "Pure Dye Calibration Run Complete".
4. Click OK to exit.
NOTE: if you wish to calibrate for more than one vessel and sealing type combination, repeat the
process above to collect additional dye calibration files. During a run the software will
automatically use the correct file for the vessel and sealing parameters specified in plate setup.
NOTE: Pure dye calibration is not required for SYBR
®
Green (dye designators “SYBR” and
“SYBR1” – “ SYBR5”).
This completes calibration of the camera on the MyiQ2 and iQ5 systems.
Fig. 7.5. The Pure Dye Calibration Window.