Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 113)

Section 6 Data Analysis Module
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dominant inter-run calibrator. The average dC
T
value of the dominant inter-run calibrator will be
used to adjust all C
T
values within the Gene Study.
To find the dominant inter-run calibrator, the iQ5 Inter-run Calibration algorithm first calculates
the average of the dC
T
values for all inter-run calibrators of a given gene. The iQ5 software uses
a multi-tiered algorithm to determine the dominant inter-run calibrator.
The algorithm utilizes the following hierarchy for determining the dominant inter-run calibrator:
1. Set the dominant calibrator to the gene with the highest number of common replicate
groups in a given pair-wise comparison.
If multiple genes have the same number of common replicate groups, then:
2. Set the dominant calibrator to the gene with the smallest range of dC
T
values in pair-wise
comparisons. The range is examined by comparing the absolute value of the difference
between the maximum and minimum dC
T
for the inter-run calibrators of a given gene.
If multiple genes have an identical range of dC
T
values, then:
3. Set the dominant calibrator to the gene with the smallest absolute value of “Average
dC
T
” of eligible inter-run calibrator samples.
If multiple genes have identical “Average dC
T
” absolute values, then:
4. Set the dominant calibrator to the replicate group with the smallest dC
T
.
NOTE: The first data file imported into the Gene Study will always serve as the “hub” file for
pair-wise data comparisons during Inter-run Calibration.
Fig. 6.61. The Inter-Run Calibrator Calculation Display.