Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 137)

Section 8 User Profiles
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8.1.8 Gene Expression Module Preferences
The Gene Expression module preference box (Figure 8.9) allows you to predetermine several
analysis and display options associated with analyzing C
T
values for gene expression analysis.
Fig. 8.9 Defining Analysis and Display Options for the Gene Expression Module.
•
Relative to: This option allows you to present data with axes originating at 1 (relative to
control) or at zero (relative to zero).
•
X Axis: This option allows you to graph either genes or conditions on the x-axis.
•
Y Axis: This option allows you to display the graph with the y-axis in a log2 scale or on a
linear scale.
•
Scaling: “Scale to control” is another option, which is accomplished by assigning a
control in the Condition List.
•
Method: These options allow you to set the default analysis mode for your gene
expression analysis. You can select ddCt (for normalized expression) or dCt (for relative
expression) as a default setting.
•
Std Devs: The default presentation for the error bars is plus and minus one standard
deviation. You can change the multiplier to get plus and minus 2 or 3 standard deviations.
8.2 User Administration
The Administration tab can be used by a user with an “iQ5 Administrator” role to:
•
Add or delete users
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Edit user details (that is, full name and Email information)
•
Set or change passwords
•
Control the permissions of each user
Only an iQ5 Administrator can access and modify information contained in the Administration
window.
Users specified as iQ5 Principal, iQ5 Operator, and iQ5 Guest have read-only access to the
Administration window, where they can review permission settings for their role.