Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 97)

Section 6 Data Analysis Module
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Setting Control Conditions
You can assign one condition as the control condition by selecting a checkbox next to the sample
in the Condition List as shown in Figure 6.44.
The iQ5 software assigns the control sample a value of 1 for every gene, and all other conditions
will be presented with values relative to this one. This makes it simple to evaluate fold expression
relative to the chosen (control) sample.
NOTE: The control condition will have a value of 1 for all genes.
To access the condition list:
1. Make sure the Settings radio button is activated.
2. Click Condition List option.
3. Select the checkbox(es) in the Ctrl column within the control condition(s) to which you
want to assign a control condition.
6.9.4.3 Data Set List
The Data Set List displays pertinent information about the data being analyzed in a given Gene
Study or .opd file. The information contained within the cells of the Data Set List is for display
only, and cannot be modified.
Each row of the Data Set List is linked to a single data tab of the gene expression plate interface,
located at the bottom of the Gene Expr window. Selecting a row from the spreadsheet
automatically toggles to its representative dye layer data tab in the plate interface. Selecting a
dye layer data tab results in toggling to its representative row in the Data Set List spreadsheet.
Information displayed in the Data Set List includes:
•
Name: Displays the fluorophore name for a given set of collected data. A number is
placed before the abbreviated fluorophore name. The assigned number helps to
distinguish between data from the same fluorophore when multiple files are used (as in a
Gene Study)
•
Full Name: This cell contains the original name of the opd file as well as the original
location of the opd when it was created
•
Created Date: This cell displays the date and time the .opd file was created
The Name and Full Name column contents can be alphabetically sorted by clicking on the title
header of each cell. This feature is helpful when long list of fluorophores are created as with
multiple files (see Gene Study).
Fig. 6.45. Accessing and viewing the Data Set List.