Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 76)

Section 6 Data Analysis Module
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The vertical temperature bar may be dragged to any position on the plot. The temperature bar
on the melt curve plot moves along to the same position as the temperature bar on the melt
peak plot is moved. If you place the cursor over a trace, so that the pointer turns into a hand,
the trace will be identified in the small box placed in the top right corner of the plot. The chart
can be expanded to fill the window by clicking the + box in the top left corner of the plot. Once
expanded, it can be returned to its original size by clicking the – box in the top left corner.
6.6.1 Melt Curve and Melt Peak Chart Menu
There are a number of features of the plot that may be modified as well as control of data
filtering. All changes specified by the context menu accessed on the melt peak plot are also
carried out on the melt curve plot. Those features are accessed by a right click on the chart,
which brings up the menu shown in Figure 6.24.
Fig. 6.24. Melt Curve/Peak Chart Context Menu.
6.6.2 Delete Selected Peaks
To remove a peak from the analysis, highlight the peak in the spreadsheet and then click Delete
Selected Peaks. You can press the Shift key to highlight multiple peaks and delete them all at
once.
6.6.3 Edit Melt Peak Begin/End Temperature
You may edit the begin and end temperatures for a melt peak by clicking Edit melt peak
begin/end temps as shown below.
After you click this option button, the currently active peak displays alone with a Begin Temp bar
and an End Temp bar. Drag the bars to the desired begin and end temperatures. As you drag the
begin and end bars along the Melt Peak plot, the iQ5 software tracks the movement on the melt
curve chart and updates the movement in the spreadsheet. When the Edit melt peak begin/end
temps option is activated, the Display Wells and Analyze Wells dialog boxes are unavailable.
In Figure 6.25, the peak begin temperature is 59°C, the peak end temperature is 79°C, and the
threshold is 1.