Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 146)

Appendices
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Appendix B: Troubleshooting Error Messages
Cause:
Selecting or creating plate setup files that contain fluorophores not compatible with the
connected instrument. The warning message above will be displayed at the bottom of the
screen. This message indicates that while the plate setup can be viewed and edited, the plate
setup contains fluorophores that cannot be used to collect new data.
Solution:
If you wish to view or edit the plate setup, no corrective action is required. If you want to run
this plate setup using the currently connected instrument, you will need to edit the plate setup to
remove fluorophores that are incompatible with the connected instrument. Refer to section 4.2
for further details
Cause:
Data files collected using an iQ5 system can be opened when the computer running iQ5 software
(version 2.1) is connected to a MyiQ2 or MyiQ system. The warning message above indicates the
plate setup contains fluorophores not compatible with the connected instrument. This message
means that while the data can be viewed and analyzed, the plate setup contains fluorophores
that cannot be used to collect new data. Similarly, opening a data file collected on the MyiQ2
system when a MyiQ system is connected to the software will result in the same message if
MyiQ-incompatible fluorophores are present in the plate setup.
Solution:
If you wish to view or edit the data, no corrective action is required. If you want to repeat the
run using the plate setup associated with the data file, you will need to edit the plate setup to
remove fluorophores that are incompatible with the connected instrument. Refer to section 4.2
and 4.5 for further details