Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 43)

Section 4 Workshop Module
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Temperature Change
You may program an automatic periodic increase or decrease in the step temperature in a
repeated cycle. Temperature increments or decrements may be as little as 0.1°C per cycle. You
may make the increase or decrease as frequently as every cycle, and the increase or decrease
can begin following any cycle. The temperature increment or decrement may be as large as
desired, as long it does not result in temperatures which are outside the temperature limits of 4–
100°C.
To program a temperature increment or decrement:
1. Click Temperature Change in the Show Options box. Three new columns will appear in
the spreadsheet.
2. For the repeated step you want to affect, enter the incremental change desired in the
Temperature change column. To decrement the temperature enter the decremental
change as a negative number (for example, –0.5).
3. Enter the repeat in which you want the change to occur for the first time in the Begin
Repeat column. Usually it is cycle 2, but it can be any cycle greater than 1.
4. Enter the frequency that you want the change to occur in the “How Often?” column.
Usually you will want the change to occur every repeat, so enter 1 in this column.
Time Change
You may program an automatic periodic increase or decrease in the step dwell time in a repeated
cycle. Time increments or decrements may be as little as 1 sec per cycle. You may make the
increase or decrease as frequently as every cycle, and the increase or decrease can begin
following any cycle. The time increment or decrement may be as large as desired, as long it does
not result in dwell times which are outside the limits of 00:00 and 99:59.
To program a time increment or decrement:
1. Click Time Change in the Show Options box. Three new columns will appear in the
spreadsheet.
2. For the repeated step that you want to affect, enter the change desired in the Time
Change column. To decrement the time, enter the decremental change as a negative
number (for example, –0:05).
3. Enter the cycle in which you want the change to occur for the first time in the Begin
Repeat column. Usually it is cycle 2, but it can be any cycle greater than 1.
4. Enter the frequency that you want the change to occur in the “How Often?” column.
Usually you will want the change to occur every repeat, so enter 1 in this column.
Cycle Description/Step Process
You can choose from a list of descriptive names, or enter one of your own to describe cycle or
step processes.
A cycle description or step process may be entered in the spreadsheet in the following manner:
1. Click Cycle Description or Step Process in the Show Options box.
2. Click the cell of the spreadsheet for the cycle or step you wish to name and either choose
one of the listed names from the pull-down menu or enter one of your own.