Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 53)

Section 5 Run-Time Central Module
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Fig. 5.3. The End Point Setpoint Box.
To begin a run, click Begin Run. The Save dialog box opens. Type a name for the optical data
file. The iQ5 software saves data automatically during the experiment.
5.5 Show Plate Window
The Show Plate window (Figure 5.4) can be used to visualize samples loaded into the reaction
block of the system. Use the Show Plate window to verify the position and orientation of samples
prior to starting a run in the MyiQ2, MyiQ or iQ5 instruments.
Fig. 5.4. The Show Plate Window.
The following steps describe typical use of the Show Plate window:
1. Collect an exposure from the camera by clicking on Expose. An image of the
fluorescence from the sample block area will be displayed.
NOTE: If the image displayed is too bright or too dim, it may be necessary to adjust the
exposure time using the pull-down box in the right hand corner of the Show Plate
window.
2. Confirm that the small green boxes are visible around each well where sample
fluorescence will need to be detected. If necessary, open the instrument and correct
sample positions to match the plate setup for sample detection.