Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 86)

Section 6 Data Analysis Module
- 77 -
Results
The Results box lists the following information:
• Source of Data: Displays the analysis mode of the source data. For End Point only data,
this is Background Subtracted. For end point analysis of PCR quantification data, the
source of data must be either PCR Base Line Subtracted or PCR Base Line Subtracted
Curve Fit. You may alter the analysis mode of the source data in the PCR Quant tab
within the Data Analysis module
•
Current method: Displays the currently selected method for assigning positive and
negative values to unknowns
•
Tolerance: Displays the currently selected mode of tolerance calculation. Immediately
beneath the displayed mode is the tolerance value. If RFUs are chosen, then the Set
Tolerance in RFUs is given. If Percentage of Range is chosen then the Calculated
Tolerance in RFUs appears
•
Range: The value displayed here is dependent on the method chosen
• If the Positives method is chosen, the Range is the Positive Controls Average RFU
minus the lowest RFU.
• If the Negatives method is chosen, the Range is the highest RFU minus the
Negative Controls average RFU.
• If the Positives & Negatives method is chosen, the Range is the Positive Controls
average RFU minus the Negative Controls average RFU.
•
+ Controls Average RFUs: Displays the average RFU of all positive controls. No value
is shown if there are no positive controls defined.
•
+ Control – Tolerance: In the Positives or Positives & Negatives methods, samples
with values equal to or higher than this amount will be called as Positive. No value is
shown if there are no positive controls defined.
•
– Controls Average RFUs: Displays the average RFU of all negative controls. No value
is shown if there are no negative controls defined.
•
– Control + Tolerance: In the Negatives method, samples with values equal to or
higher than this amount will be called as Positive. In the Positives & Negatives method,
samples with values less than this amount will be called Negatives. No value is shown if
there are no negative controls defined.
6.8.2 End Point Analysis Spreadsheet
The End Point analysis spreadsheet contains the necessary data to perform end point analysis,
and also displays the values assigned to unknowns after Recalculate is clicked. The table
headings include the following:
• Well: Lists the well ID of each sample. You may click on the Well column header to sort
the table by wells. Note that you may also include or exclude certain wells from end point
analysis via the Analyze Wells button above the spreadsheet
• Sample Type: Lists the sample type of every well, as defined in the plate setup. You
may click on the Sample Type column header to sort the table by sample type
• End RFUs: Lists the absolute RFU averages for each well, as calculated from the end
cycles, which are specified in the End Cycles to Average field. You may click on the End
RFUs column header to sort the table by end RFUs