Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 136)

Section 8 User Profiles
- 127 -
8.1.6 Allelic Discrimination Module Preferences
The Allelic Discrimination preference box (Figure 8.7) can be used to set the default display
conditions used in the Allelic Discrimination screen. The Display Mode can be set to Threshold
Cycle or RFU using the radio buttons. The radio buttons for the Normalize Data option become
active only when the Display Mode is set to RFU.
Fig. 8.7. Defining Allelic Discrimination Display Options.
8.1.7 End Point Module Preferences
The End Point module preference box (Figure 8.8) allows you to predetermine several analysis
and display options associated with analyzing final RFU in an end point analysis assay.
Fig. 8.8. Defining Analysis and Display Options for the End Point Module.
•
Method: The Method box allows you to select the method of assigning positive and
negative values to your unknowns based on RFU values. The Method box consists of the
following three choices: Negatives, Positives, and Positives & Negatives. Analysis by
comparison to negative controls (Negatives) is the default method.
•
Ranks: The number of ranks allows assignment of samples into distinct groups based on
their RFU values. The default Rank value is 10 and the minimum number of Ranks is 3.
•
End Cycles to Avg: End Cycles to Average is the number of cycles, from the last cycle,
that will be used to calculate an average end point RFU value. The End Cycles to Avg field
defaults to 5 for non-end point only (PCR) runs.
•
End Point Only Run: The End Cycles to Avg field defaults to 2 for end point only runs.
•
Tolerance Mode: End Point Tolerance defines the margins for sorting unknowns as
positives or negatives. The End Point Tolerance drop-down list box consists of two
choices: RFUs and Percent of Range.
•
Tolerance %: Select a value between 1 and 99 percent for this setting. The default
percent of range tolerance is 10 percent.
•
Color Scheme: Colored rank boxes symbolize the number and order of ranks in the end
point analysis. There are five options to choose from that allow a change in the color
scheme of the rank boxes, once the data are analyzed.