Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 125)

Section 7 Calibrating the Instrument
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To generate persistent well factors:
1. Select a well sealing type (film or caps) from the drop-down list.
2. Select a vessel type (plates or tubes) from the drop-down list.
3. Click Collect Persistent Well Factors.
4. When the iQ5 system completes the persistent well factor calibration run, a dialog box
appears with the message, "Persistent Well Factor Calibration Run Complete".
5. Click OK to exit.
NOTE: If you wish to calibrate for more than one vessel and sealing type combination, repeat the
process above to collect additional external well factor files. During a run, the software will
automatically use the correct file for the vessel and sealing parameters specified in plate setup.
This completes the generation of persistent well factors. This completes calibration of cameras on
the MyiQ system. To calibrate the cameras on the MyiQ2 and iQ5 systems, perform pure dye
calibration as described in section 7.4.4.
Fig. 7.4. The Well Factors Window.
7.4.4 Performing Pure Dye Calibration
Before you perform a pure dye calibration run, complete the following steps:
1. Prepare a pure dye calibration plate (see section 7.3).
2. Ensure that the mask has been aligned.
3. Ensure that background calibration has been performed.
4. Ensure that persistent well factors have been generated.
5. Place the pure dye calibration plate in the iCycler thermal cycler.
6. Select the Pure Dye tab in the Calibration module.
The Pure Dye Calibration window is shown in Figure 7.5.