Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 147)

Appendices
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Cause:
This error appears when the software/computer is unable to communicate with the camera.
The iQ5 software and camera are on but the iCycler base unit is powered off.
Solution:
Close the software, turn the camera on and restart the iQ5 software.
Check that the USB cable is securely connected to both the camera and computer.
Ensure that the 2.0 high-speed enabled USB port on the computer is being used and all hibernate
and power save settings are disabled. Refer to section 1.2.5 and 1.4 for further details.
Cause:
Background factor data not found. This error occurs when a run start is attempted using a vessel
and seal combination the instrument has not been calibrated for.
Solution:
The background vessel and seal type selected for your plate setup must match collected
background calibration files. The instrument should ideally be re-calibrated for the desired vessel
and seal type combination. If a run must be started immediately, select a vessel and seal type for
which the instrument has already been calibrated. After the run has completed, recalibrate the
instrument for the desired vessel and seal combination and back-apply this new background
calibration to your data file. Refer to section 4.5.5 for further details.