Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 6)

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5.5 Show Plate Window .....................................................................................................44
5.6 Monitor Run Window....................................................................................................45
5.7 Run-Time Protocol Editing ............................................................................................45
Section 6. Data Analysis Module ..................................................................................47
6.1 PCR Quant Tab ............................................................................................................48
6.1.1 Customizing the PCR Quant Display ........................................................................49
6.2 Amplification Chart.......................................................................................................49
6.2.1 Fluorophore Selector Buttons..................................................................................49
6.2.2 Select Analysis Mode..............................................................................................50
6.2.3 Log View Button ....................................................................................................50
6.2.4 Selecting and Viewing Traces .................................................................................51
6.3 Standard Curve Chart ...................................................................................................53
6.3.1 Standard Curve Chart Menu....................................................................................54
6.4 Results Section ............................................................................................................55
6.4.1 Plate Spreadsheet ..................................................................................................55
6.4.2 Standard Curve/C
T
Results Spreadsheet ..................................................................56
6.4.3 Amplification Data (RFU) Spreadsheet.....................................................................57
6.5 Amplification Plot Context Menu....................................................................................57
6.5.1 Data Analysis Options ............................................................................................57
6.5.2 Amplification Plot Data Display Options ...................................................................61
6.5.3 Data Export Options...............................................................................................64
6.5.4 Exporting Results to Microsoft Excel ........................................................................64
6.5.5 Printing Results......................................................................................................65
6.6 Melt Curve and Melt Peak Charts...................................................................................65
6.6.1 Melt Curve and Melt Peak Chart Menu.....................................................................67
6.6.2 Delete Selected Peaks ............................................................................................67
6.6.3 Edit Melt Peak Begin/End Temperature ...................................................................67
6.6.4 Melt Curve/Peak Control Area .................................................................................68
6.6.5 Melt Curve/Peak Spreadsheet .................................................................................68
6.7 Allelic Discrimination Module (For Multiplex Data Only)...................................................69
6.7.1 Allelic Discrimination Plot........................................................................................69
6.7.2 Allelic Discrimination Plot Menu...............................................................................71
6.7.3 Allelic Data Spreadsheet.........................................................................................71
6.7.4 Automatic/Manual Call ...........................................................................................72
6.7.5 Manual Calls ..........................................................................................................73
6.7.6 Display Mode.........................................................................................................73