Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 106)

Section 6 Data Analysis Module
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Standard Deviation for the Normalized Expression
Rescaling this value is accomplished by dividing the standard deviation of the normalized
expression by the normalized expression value for the highest or lowest expresser depending on
which scaling option you choose.
When a control is assigned you need not perform this rescaling function on the standard
deviation as illustrated below.
Standard Deviation of the Scaled Normalized Expression
Rescaling this value is accomplished by dividing the standard deviation of the normalized
expression by the normalized expression value for the highest or lowest expresser depending on
which scaling option you choose.
When a control is assigned, you need not perform this rescaling function on the standard
deviation.
6.9.9 Relative Quantity Calculations
Relative Quantity Calculations with No Controls Identified
Relative Quantity (dCT) for any sample for gene x is calculated as follows.
Where E = Efficiency of primer (primer/probe) set
this efficiency is calculated as follows (% Efficiency * 0.01 + 1)
where 100% = 2
C
T (MIN)
= Average C
T
for the sample with the lowest average C
T
for gene x
C
T (sample)
= Average C
T
for the sample