Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 110)

Section 6 Data Analysis Module
- 101 -
6.10.2 Creating a Gene Study File
A Gene Study file must contain data from at least two different .opd files. The process of creating
a Gene Study file is a 2-step process.
STEP 1: Prepare .opd files for gene expression analysis.
1. In the PCR Quant tab, establish the desired experimental conditions, such as baseline
and threshold and the wells to be included in the analysis.
NOTE: Additional wells can be excluded or re-included later from the gene expression
plate interface.
2. Click on the Gene Expr tab. Make sure that all files to be included in the Gene Study have
the Enable for Gene Study button selected. The default setting is that this button is
not enabled.
3. After enabling the file for multi-file analysis, save the .opd file.
4. Repeat steps 1–3, above, for all additional files to be included in the Gene Study.
STEP 2: Add files to a new Gene Study.
1. From the menu toolbar, select File > New > Gene Study. The Gene Expression Study
Manager will be displayed.
2. Select Add .opds. The Windows file explorer dialog box will appear.
3. Locate and select the .opds needed for the Gene Study. Multiple files may be added to
the Gene Study at one time.
4. Click OK when finished.
Once the files are added to the Gene Expression Study Manger, selecting OK opens an unsaved
Gene Study file in the Gene Expression window of the iQ5 software. The Gene Study, which
contains all the files selected from the Gene Expression Study Manager, can now be analyzed as
a normal gene expression file by selecting the analysis method, assigning Gene and Condition
Names from the gene expression plate interface and assigning attributes from the Gene List.
NOTE: Gene Study files are NOT automatically saved at the time of file creation. It is highly
recommended to save the newly created .gxd file after initiating a Gene Study.
When the files are added to the Gene Expression Study Manager, the file information is
separated into four columns:
• File Selection: This column is used in conjunction with the Remove button, and
features a checkbox in the column header. The “header” checkbox will select or deselect
all the displayed files chosen for the Gene Study. To select or deselect an individual file,
click the checkbox within the corresponding row. If one or more files have been selected,
clicking Remove will remove those file(s) from the Gene Study.
•
File Name: This column displays the full name of the .opd files to be included in the
Gene Study.
•
File Directory: This column displays the directory location of the .opd files to be
included in the Gene Study.