Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 124)

Section 7 Calibrating the Instrument
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3. Place the background calibration plate in the iCycler thermal cycler.
4. Select the Background tab in the Calibration module.
The Background window is shown in Figure 7.3.
Fig. 7.3 The Background Window.
To perform background calibration:
1. Select a well sealing type (film or caps) from the drop-down list.
2. Select a vessel type (plates or tubes) from the drop-down list.
3. Click Collect Background Readings.
4. When the iQ5 software completes the background calibration run, a dialog box appears
with the message, "Background Calibration Run Complete."
5. Click OK to exit.
NOTE: If you wish to calibrate for more than one vessel and sealing type combination, repeat the
process above to collect additional background calibration files. During a run the software will
automatically use the correct file for the vessel and sealing parameters specified in plate setup
This completes background calibration. Next, perform persistent well factor calibration.
7.4.3 Generating Persistent Well Factors
Before you perform a persistent well factor calibration run, complete the following steps:
1. Prepare an external well factor plate (see section 7.3)
2. Ensure that the mask has been aligned and that background calibration has been
performed.
3. Place the external well factor plate in the iCycler cycler.
4. Select the Well Factors tab in the Calibration module.
The Well Factor window is shown in Figure 7.4.