5 data file – Bio-Rad iQ™5 Optical System Software, Version 2.1 User Manual

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Section 4 Workshop Module

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Fig. 4.17. The Run Set Selection Window.

4.4.1 Selecting a Run Set

1. Click Run Set.

2. Use the browser below the Run Set button to locate the folder containing the run set file

, and then click on the file name to select it.

3. The selected run set will appear in the Selected Protocol and Selected Plate Setup

windows.

4.4.2 Creating a Run Set

To create a run set:

1. Select the plate setup and protocol that are to be linked.

2. The plate setup and protocol can be stand-alone files (plate setup files have the

extension .pts while protocol files have the extension .tmo) or they can be already
together in a data file (with the extension .opd). A data file has two plate setups: Original
(the Plate Setup the data file was created with) and Current (the plate setup the data file
was last saved with). The run set will be created with the plate setup displayed in the
Selected Plate Setup window.

3. Once you have chosen the protocol and plate setup files, from the File toolbar menu,

select New, Run Set and enter a name for your new run set.

4.5 Data File

This feature is primarily used to select and open a data file. It can also be used to run a real-time
experiment using the same protocol and plate setups that were used to create the data file. The
plate setup that is selected can be either the Original or Current (last saved) plate setup.

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