Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 19)

Section 2 Quick Guides
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2. From the Workshop Setup window, select your desired Plate (.pts) and Protocol (.tmo)
file individually, or your desired Run Set (.run).
3. Click Run.
4. Check that the desired Protocol and Plate Setup are displayed in the bottom half of the
Initiate Run screen.
5. Select the type of well factors to use by selecting either:
•
Use Persistent Well Factors
or
• Collect Well Factors from Experimental Plate
6. Click Begin Run.
7. Name the file with a unique name in the Save Optical Data File dialog box.
8. Click OK.
2.3.2 Monitoring the Run
When the real-time PCR detection system begins the run, the iQ5 software opens the Monitor
Run window. You can see the progress of the run in this window.
At the end of the run, the Run Status dialog box appears. You can choose between displaying the
data in the Data Analysis module or returning to the Workshop module.
Click Yes to proceed to the Data Analysis module.
2.4 Data Analysis Quick Guides
When the iQ5 software opens, the Data Analysis module is grayed out and inactive. To analyze a
data file, click the Data File tab of the Workshop module, select the data file, and then click
Analyze.
The Data Analysis module consists of six tabs:
• PCR Quant
• Melt Curve/Peak
•
End Point
•
Allelic Disc
• Gene Expr
• Edit Plate
2.4.1 PCR Quant Tab Quick Guide
Use the PCR Quant tab to set the analysis conditions for the data file including setting the PCR
baseline, setting the threshold and determining which wells to exclude or include in the
experiment.
To analyze a data file:
1. Click the Data File tab in the Workshop module.