Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 50)

Section 5 Run-Time Central Module
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Section 5. Run-Time Central Module
This section contains information of the following topics:
o
Initiating a Run (page 41)
o
Well Factors (page 42)
o
Real-Time PCR experiments using binding dyes (page 43)
o
Running End Point experiments (page 43)
o
Monitoring a Run (page 45)
o
Run-Time Protocol Editing (page 45)
The Run-Time Central module is entered automatically after clicking Run or Run End Point from
the Workshop Setup window (Figure 5.1). There are three tabs within the Run-Time Central
module: Initiate Run, Show Plate and Monitor Run.
Fig. 5.1. Accessing the Run-Time Central Module.
5.1 Initiate Run Window: Run Selected
Use the Initiate Run window (Figure 5.2) to confirm run conditions and then to initiate the optical
data run. The protocol to be run is in the bottom left corner of the window, and the plate setup
that will be used appears in the bottom right corner. The type of well factors to be used in the
run can be selected in the top left section of the window. Record details of the experiment in the
Notes box. These notes will be incorporated into the experimental file.
To begin a run, select your desired well factor collection method and click Begin Run. The Save
dialog box opens. Type a unique name for the optical data file. The iQ5 software saves data
automatically during the experiment.