Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 135)

Section 8 User Profiles
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Last File Used: This option will select the last Protocol, Plate Setup, Run Set or Data File
used as the file to display in the Workshop screen when the iQ5 software starts up
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Data File: If you wish to have a specific data file selected when the iQ5 software opens,
select Data File and then use the browse button to navigate the data file that you wish to
have selected on application startup
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Run Set: If you wish to have a specific Run Set file selected when the iQ5 software
opens, select Run Set and then use the browse button to navigate the run set file that
you wish to have selected on application startup
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Protocol/Plate: If you wish to have a specific protocol or plate setup file selected when
the iQ5 software opens, select Protocol/Plate and then use the browse button to navigate
either the protocol or plate file that you wish to have selected on application startup
8.1.5 PCR Quant Screen Preferences
The PCR Quant preference box (Figure 8.6) can be used to set the defaults for the PCR Quant
screen. You can set the defaults for four major settings that will impact the analysis or display of
data collected from your real-time PCR detection system.
Fig. 8.6. Defining User Specific PCR Quant Analysis and Display Options.
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Baselining: By default, PCR baselines are auto-calculated. With the Baselining user
preference setting, you can override auto-calculation by clicking User Defined. After you
click User Defined, the iQ5 software activates the User Defined Baseline Cycle Range
boxes. Enter the desired values for the start cycle and ending cycle for all traces. Auto
Calculated is the factory default
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Log View: Use this option to select either a semi-logarithmic or linear view of the PCR
amplification chart data. When the Log View option is On, a semi-logarithmic view of the
amplification data will be displayed. The factory default for Log View is Off
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Analysis Mode: You can select from three analysis modes in the Analysis Mode drop-
down list boxes. The three analysis modes include: Background Subtracted, PCR Base Line
Subtracted, PCR Base Line Subtracted Curve Fit. PCR Base Line Subtracted Curve Fit is the
factory default
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Grid Rows: The Grid Rows options are a set of checkboxes that you can use to display
additional details about the data displayed in the PCR Quant screen. These optional details
will be displayed in the results spreadsheet of the PCR Quant screen, and include the
following information about each sample: Identifier, Concentration, Threshold Cycle, and
End Point Call