2 standard curve/c, Results spreadsheet – Bio-Rad iQ™5 Optical System Software, Version 2.1 User Manual

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Section 6 Data Analysis Module

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Fig. 6.10. The Plate Spreadsheet.

The spreadsheet displays only one fluorophore at a time. You can use the Plate spreadsheet
fluorophore selector buttons found under the Plate spreadsheet to determine which fluorophore
is displayed in the spreadsheet. Each well has a colored bar at the top of the cell, which is the
color used in the amplification chart to display the amplification trace for this well and
fluorophore. The sample type is always displayed in this spreadsheet.

Above the spreadsheet is a set of checkboxes you can use to display the Identifier,
Concentration, Threshold Cycle, and End Point Calls in the Plate spreadsheet.

6.4.2 Standard Curve/C

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Results Spreadsheet

The Standard Curve/C

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Results spreadsheet displays the well, fluorophore, sample type,

identifier, replicate number, threshold cycle, starting quantity, and statistics for replicate groups.

Access the Standard Curve/C

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Results spreadsheet by clicking Results on the PCR Quant screen.

By default the Standard Curve/C

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Results tab will open displaying the spreadsheet results (Figure

6.11).

Fig. 6.11. Standard Curve/C

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Results Spreadsheet.

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