Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 142)

Section 9 Instrument Maintenance
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Section 9. Instrument Maintenance
This section contains information on the following topics:
o
Cleaning the system (page 133)
o
Recommended fluorophores and filter specifications (page 133)
o
Accessing and cleaning the filters (page 134)
o
Replacing the lamp (page 135)
9.1 Cleaning the Real-Time PCR Detection System
Take care not to spill liquids onto or into the iCycler thermal cycler or the optical module. Cleaning
may be done using a lint-free cloth or paper towel. The case may be cleaned using a soft, lint-free
cloth and water.
9.2 Filter Description and Installation Instructions
The filters designed for use in the MyiQ2, MyiQ and iQ5 optics modules are made of glass and
mounted in plastic holders. The filter holders are held in either the excitation or emission filter
wheel of the optics module. Each filter wheel holds six filters. Every position in a filter wheel must
have a filter or an opaque filter blank to avoid damage to the CCD detector. The first position in
each filter wheel is designated as the "home" position and must always contain an opaque filter
blank. Filters can be removed for cleaning or replacement. If a filter shatters or breaks during the
installation process, contact your local Bio-Rad office immediately for service; do not attempt to
remove the broken components from the interior of the camera housing.
It is critically important that the excitation and emission filters are in the correct positions in the
filter wheels. Please confirm that the filters are in the proper location after cleaning or replacing
filters in the optics module. Section 9.2.1 summarizes the positions of the filter pairs for the
systems, optical characteristics, and the recommended fluorophores with which the filters are
compatible.
9.2.1 Recommended Fluorophores and Filter Specifications
Recommended fluorophores for the MyiQ2 Real-Time PCR Detection System
• Filter position 2
485/30X 530/30M Fluorescein (FAM), SYBR ® Green I
• Filter position 3 530/30X 575/20M HEX, TET, VIC, JOE
Recommended fluorophores for the MyiQ Real-Time PCR Detection System
• Fluorescein (FAM), SYBR
® Green I
Recommended fluorophores for the iQ5 Real-Time PCR Detection System
• Filter position 2 485/30X 530/30M Fluorescein (FAM), SYBR ® Green I
• Filter position 3 530/30X 575/20M HEX, TET, VIC, JOE