Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 67)

Section 6 Data Analysis Module
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Fig. 6.13. The Set Data Analysis Window.
To set the Data Analysis Window:
1. Right-click on the PCR amplification plot, and click Set Data Analysis Window in the
menu.
2. Click either the Beginning of Cycle or End of Cycle option to select data from the
beginning of the cycle or the end of the cycle, respectively.
3. Set the window width in the Set Window Width scroll box.
4. To use all data points in the cycle, select Use Full Cycle Scan.
5. Click OK to return to the PCR Quant screen.
NOTE: To center the analysis around the data collected with a window in the middle of the cycle,
select Set Window Center and Width. Use the up or down arrows of the Set Window Width scroll
box to select the percentage of data points. These data points will be chosen around the value
set in the Set Window Width (% Full Cycle) text box. Click OK to return to the PCR Quant screen.
Digital Filter
After you click Digital Filter in the amplification plot menu, the Set Digital Filters window appears
(Figure 6.14). There are two intra-cycle data filtering options available: Rolling Boxcar and
Weighted Mean. The default filter is the weighted mean, as this filter is the only one available
during data acquisition.
Fig. 6.14. The Set Digital Filters Window.
The weighted mean is determined by the equation:
O
i
= (R
i
+ c*M)/(1 + c)