Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 134)

Section 8 User Profiles
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IMPORTANT NOTE: The Fluorophores preference box will display all fluorophores available to
the MyiQ2, MyiQ and iQ5 systems. If both dyes that can and cannot be detected by the connected
instrument are selected in user preferences, only fluorophores that are compatible with the
connected instrument will be displayed. If only fluorophores that are not available for detection by
the connected instrument are selected, by default the Plate Setup window will display FAM only.
8.1.3 Protocol Preferences
The Protocol preferences box can be used to set the defaults for the current user for Melt Curve,
Gradient, and Well Factor Collection Type (Figure 8.4).
Fig. 8.4. Defining User-Specific Protocol Preferences.
The following default conditions can be set for the Protocol:
•
Melt Curve: Enter the desired beginning temperature (Set Point), ending temperature
(End Temp), and the change in temperature that will occur at each repeat (Temp
Change). Click Save
•
Gradient: Enter the desired lowest temperature (Set Point) and the Range for the
gradient in the appropriate text boxes (or use the scroll buttons to enter these values).
Click Save
•
Well Faction Collection Type: Select the radio button associated with the well factor
that you wish to use as the default type. Click Save. If Dynamic is chosen as the default
type, dynamic well factors will be used unless restricted by the Plate Setup
8.1.4 File Selection at Application Startup Preferences
The file that you wish to have automatically selected when the iQ5 software is opened is
determined using the File Selection at Application Startup radio buttons (Figure 8.5).
Fig. 8.5. Selecting Custom File Defaults for Application Startup.