Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 26)

Section 2 Quick Guides
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8. Click a sample type icon.
9. Select the type of replicate loading desired.
10. Click or drag across the plate to define wells with the selected fluorophore and sample
type.
11. Continue defining the remaining wells that will contain the first fluorophore by changing
to other sample type icons as appropriate.
To calculate standard concentrations automatically, click Dilution Series and enter the
upper or lower concentrations and units of the standards, set the dilution factor, and
click Apply Dilution Series.
12. Repeat steps 7–11 for any additional dye layers/fluorophores as required. Remember
that if the Whole Plate Loading box is checked, changes made in standard concentrations
will be applied to all dye layers for that well and extended to all replicates in the group.
13. Click Apply Plate Changes to make the changes. To see the effect on analysis, go to one
of the other Data Analysis windows.
NOTE: To delete a previously defined well, click the Delete All icon, and then click the
well. To delete the selected fluorophore from a previously defined well, click the Delete
Fluorophore icon, and then click the well.
NOTE: Use the Next # checkbox to enter a particular number to assign to the next
standard or sample you define.
NOTE: The original plate setup is retained with the data file and may be restored at any
time by clicking Restore Original Plate.