Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 35)

Section 4 Workshop Module
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4.2.5 Whole Plate Loading
There are two pieces of information required for each well: sample type and fluorophore(s) to be
monitored. There are two ways of providing that information to the plate setup editor.
When Whole Plate Loading is selected, you specify the sample type and fluorophores separately.
•
In Whole Plate Loading, changes made to any fluorophore within a well are extended to the
other fluorophores within the well upon pressing Enter or clicking in another cell of the
spreadsheet. These changes are then extended to every other well within the replicate
group. This is the most efficient way of defining a plate setup for a multicolor experiment
when all wells have the same sample type at each dye layer (monitored fluorophore). For
example, you might choose to monitor four fluorophores in well A1, and in each dye layer the
sample type is Unknown. Note that if you choose Whole Plate Loading, it is assumed that
standards in each dye layer are at the same concentration, that is, the FAM standard might
be at 500 copies and so, by definition, the HEX standard is also at 500 copies.
•
When the sample types vary within a well, or in the special case of standards, when the
concentration of standard varies with the dye layer, deselect Whole Plate Loading. When
Whole Plate Loading is deselected, you specify the type of sample and fluorophore
simultaneously, one dye layer at a time.
• When Whole Plate Loading is toggled off, changes made to one fluorophore are considered
unique to the fluorophore and are not automatically extended to the other fluorophores in
the well. However, these changes are extended in the same fluorophore to every other well
within the replicate group.
4.2.6 Specifying Replicates
The buttons for specifying replicates run down the left side of the representation of the
experimental plate.
This is the default setting and indicates that each sample is identical (that is, a
single replicate). With this button active, if you:
•
Click an unused well, the sample type is incremented by one
•
Click a row letter or column number, the sample type for every
member of the row or column is assigned the identical replicate
number
•
Drag across a selection, the sample type for every member within the
selection is assigned the identical replicate number