Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 98)

Section 6 Data Analysis Module
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6.9.5 Applying an Analysis Method
1. Choose an analysis method by clicking one of the option buttons:
•
Normalized expression (ddCt) is the default and requires you to first choose a
reference gene)
or
• Relative quantity (dCt)
2. Click on Recalculate to apply the new settings.
NOTE: Many changes made to the plate setup and analysis options require that you
recalculate. If you do not immediately see the change you expect to see, click
Recalculate (Figure 6,46).
Fig. 6.46. Applying an Analysis Method.
6.9.6 Data Table for Gene Expression Analysis
The Data Table spreadsheet for gene expression analysis is accessed by clicking Data Table. The
Data Table spreadsheet lists the Condition and Gene names, and calculated expression and C
T
values (Figure 6.47). Columns can be resized and re-ordered by clicking and dragging. Any
condition being used as a control is indicated by an asterisk in the Ctrl column. Columns can be
sorted by Condition or Gene name by clicking on the sort triangle icon next to the column
heading.
Fig. 6.47 Gene Expression Analysis Data Table.