Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 118)

Section 6 Data Analysis Module
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• PCR Quant Detailed: This report template includes all of the data available in the PCR
Quant module of the iQ5 software – including the PCR amplification chart, C
T
value
spreadsheet, and analysis parameters. If the dataset being analyzed also contains a
standard curve, the standard curve chart and spreadsheet data will also be included in this
report.
•
PCR Quant Graph Only: This report template displays only the PCR amplification chart,
exactly as analyzed and formatted within the PCR Quant module.
•
Std Curve Data Only – Landscape: This report template includes only the data included
in the Standard Curve/C
T
Results spreadsheet. Although the report on the screen in
displayed in the Portrait orientation, the Page Layout print settings are pre-set to the
Landscape orientation.
Melt Curve Reports
•
Melt Curve Detailed: This report template includes all of the data available in the Melt
Curve/Peak module of the iQ5 software – including the melt curve chart, melt peak chart,
Melt data spreadsheet, and analysis parameters. In addition, if all fluorophores have been
selected for display in the PCR Quant module, the PCR amplification chart and analysis
parameters will also be displayed.
•
Melt Curve Graph Only: This report template displays only the graphs available in the
Melt Curve/Peak module of the iQ5 software, such as the melt curve chart and melt peak
chart, exactly as analyzed and formatted within the Melt Curve/Peak module.
•
Melt Curve Only: When creating a report from a data set performed as an Melt Curve
Only run, the Melt Curve Only report option becomes available. This report template
includes all of the data available in the Melt Curve/Peak module of the iQ5 software –
including the melt curve chart, melt peak chart, Melt spreadsheet, and analysis parameters.
End Point Reports
•
End Point detailed: When creating a report from a data set that includes PCR
Quantification data, End Point detailed is the only report option available. This report
includes a section titled “Data Analysis Parameters”, which details the PCR analysis settings
for the current dataset.
•
End Point only: When creating a report from a data set performed as an End Point Only
run, the End Point Only report is the only report option available. This report template
contains only the End Point Analysis spreadsheet and data analysis parameters.
Allelic Disc Reports
•
Allelic Data Only: The Allelic Data Only report template is limited to the data displayed in
the Allelic Disc module only, but will not display the allelic discrimination chart.
•
Allelic Detailed: This report template includes all of the data available in the Allelic Disc
module of the iQ5 software – including the allelic discrimination chart, spreadsheet, and
analysis parameters. In addition, if all fluorophores have been selected for display in the
PCR Quant module, the PCR amplification chart and analysis parameters will also be
displayed.
•
Allelic Only: This report template is limited to the data displayed in the Allelic Disc module
only and includes the allelic discrimination chart, spreadsheet, and analysis parameters.
Gene Expression Reports
•
Gene Expression Detailed: This report template includes all the Gene Expression data
available in the Gene Expr module, including charts, data tables, Gene List and Condition
List information, Threshold Crossing Spreadsheet, and Gene Expression analysis