Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 37)

Section 4 Workshop Module
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Fig. 4.8. The Seal and Vessel Type Dialog Box.
4.2.8 Defining a Dilution Series
The iQ5 software can automatically calculate the concentrations of each member of a dilution
series, given the dilution factor and the concentration of either the most or least concentrated
sample (Figure 4.9).
Fig. 4.9. The Dilution Series Dialog Box.
1. Access the dilution series box by clicking on Dilution Series.
2. Define the range of standard replicates to be defined. By default, the beginning and
ending standards are the lowest- and highest-numbered standards, but you may define
only a subset of all standards by clicking on the up or down arrows.
3. Enter the starting concentration, the concentration of the lowest-numbered standard to
be defined. The Scientific Notation button controls only how the data are displayed in the
spreadsheet.
4. Enter a series identifier for the standard if desired. It will be shown in the spreadsheet
and in the reports.
5. Enter the fold dilution factor. The default value is 10.
6. Indicate whether the concentrations decrease or increase as the standard replicates
increase in number. The default setting is Decreasing.
7. Click Apply Dilution Series, and all standard concentrations will be automatically
calculated.
Adding Additional Standards
When Define is selected (at the bottom of the dialog box), standards added to the set after the
Dilution Series box is closed will have their concentrations automatically calculated.