Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 25)

Section 2 Quick Guides
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• Change the condition assignments in these wells by typing your desired name into
the condition pull-down menu, then click enter to apply the name to the selected
wells
NOTE: The Gene and Condition Names have a character entry limit of 15 characters.
•
Minimize the Gene Expression Plate interface by clicking on the “–” button to return
to the standard view of the Gene Expr tab window
7. Select either Normalized expression (ddCt) or Relative quantity (dCt).
NOTE: For Normalized Expression analysis you must first assign reference gene(s). Click
the Settings tab, then select Gene List spreadsheet to set your desired reference gene(s).
8. Click Recalculate to see your results.
9. Normalized Expression results are graphed. The Data Table spreadsheet is accessed by
clicking Data Table. The Data Table spreadsheet lists the Condition and Gene name,
calculated expression values and C
T
values. Right-click on the spreadsheet to print or
export this data to Excel.
NOTE: Click the Settings tab, then select Gene List to enter a specific user-defined gene
reaction efficiency.
NOTE: Use the Settings tab, then select Condition List to select a particular sample as a
control sample.
2.4.5 Post-Run Plate Setup Editing Quick Guide
For post-run editing of the Plate Setup saved within a data file:
1. From the Workshop module:
•
Click Data File above the directory of the home workshop. Navigate the directory
until the desired data file is found. Double-click the file name to bring the file
directly into the Data Analysis module
or
• Click Data File above the directory of the home workshop. Navigate the directory
until the desired data file is found. Click the file name once to open the plate
setup associated with the data file in the bottom right section of the Workshop
window. Click Analyze to bring the data to the Data Analysis module
2. At the top of the Data Analysis window, click Edit Plate.
3. A modified version of the Plate Setup Editor will open. In this modified window, you
cannot add fluorophores to or remove fluorophores from the fluorophore list. Nor may
you add a previously defined fluorophore or remove a previously defined fluorophore
from a well.
4. Edit any notes about the plate setup in the Notes box.
5. Edit the name of the experiment in the Experiment Name box.
6. For most experiments the Whole Plate Loading box will be checked. With Whole Plate
Loading, changes made to any fluorophore within a well are extended to the other
fluorophores within the well and within the replicate group. If you are editing a plate, the
Whole Plate Loading checkbox may be unavailable because it is not appropriate based on
the current definition of the plate.
7. Click a fluorophore.