Bio-Rad iQ™5 Optical System Software, Version 2.1 - Manual (Page 5)

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4.1.4 Selected Data File Area ..........................................................................................21
4.2 Plate Setup ..................................................................................................................21
4.2.1 Plate Setup Editor Window .....................................................................................21
4.2.2 Well Definition Icons ..............................................................................................22
4.2.3 Editing or Creating a New Plate Setup .....................................................................23
4.2.4 Fluorophore Selection ............................................................................................24
4.2.5 Whole Plate Loading ..............................................................................................26
4.2.6 Specifying Replicates..............................................................................................26
4.2.7 Sample Volume, Seal Type, and Vessel Type ...........................................................27
4.2.8 Defining a Dilution Series .......................................................................................28
4.2.9 Plate Setup Editor Spreadsheets .............................................................................29
4.2.10 Plate Summary ....................................................................................................30
4.3 Protocol.......................................................................................................................31
4.3.1 Selecting a Protocol ...............................................................................................31
4.3.2 Editing or Creating a Protocol .................................................................................32
4.3.3 Add Protocol Options .............................................................................................33
4.3.4 Gradient ................................................................................................................35
4.3.5 Melt Curve/Peak ....................................................................................................36
4.3.6 Sample Protocol Files .............................................................................................37
4.4 Run Set .......................................................................................................................37
4.4.1 Selecting a Run Set................................................................................................38
4.4.2 Creating a Run Set.................................................................................................38
4.5 Data File......................................................................................................................38
4.5.1 Selecting a Data File ..............................................................................................39
4.5.2 Opening a Data File ...............................................................................................39
4.5.3 Opening a Pure Dye Calibration file .........................................................................39
4.5.4 Applying Alternate Pure Dye Calibrations (Alternate RME) ........................................39
4.5.5 Applying Alternate Background or Well Factors ........................................................40
Section 5. Run-Time Central Module............................................................................41
5.1 Initiate Run Window: Run Selected ...............................................................................41
5.2 Well Factors.................................................................................................................42
5.2.1 Dynamic Well Factors.............................................................................................42
5.2.2 Persistent Well Factors ...........................................................................................42
5.3 Real-Time PCR Experiments Using DNA-Binding Dyes.....................................................43
5.3.1 Spiking Real-Time PCR Experiments Using DNA binding Dyes With Fluorescein..........43
5.4 Initiate Run Window: Run End Point Selected ................................................................43